3GPN
Structure of the non-trimeric form of the E113G PCNA mutant protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-04 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.072 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.590, 147.509, 81.442 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.380 - 2.500 |
| R-factor | 0.23636 |
| Rwork | 0.234 |
| R-free | 0.27340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1plq |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.676 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.380 | 2.565 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.365 | |
| Number of reflections | 14864 | |
| <I/σ(I)> | 11.4 | 3.2 |
| Completeness [%] | 98.2 | 96.79 |
| Redundancy | 4.75 | 4.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 291 | 1.6 M ammonium sulfate and 0.1 M sodium Citrate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
