3GPM
Structure of the trimeric form of the E113G PCNA mutant protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-08 |
| Detector | NOIR-1 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 122.093, 122.093, 122.093 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.620 - 3.800 |
| R-factor | 0.27531 |
| Rwork | 0.273 |
| R-free | 0.31215 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1plq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.376 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.620 | 3.903 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.294 | |
| Number of reflections | 6146 | |
| <I/σ(I)> | 17.9 | 6.8 |
| Completeness [%] | 99.6 | 99.57 |
| Redundancy | 10.56 | 9.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 2.0 M ammonium sulfate and 0.1 M sodium citrate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
