3GPB
COMPARISON OF THE BINDING OF GLUCOSE AND GLUCOSE-1-PHOSPHATE DERIVATIVES TO T-STATE GLYCOGEN PHOSPHORYLASE B
Experimental procedure
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.500, 128.500, 116.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| Rwork | 0.181 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 3.500 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.300 * |
| Rmerge | 0.066 * |
| Total number of observations | 89325 * |
| Number of reflections | 37461 * |
| Completeness [%] | 88.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.7 * | unknown * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 30 (mg/ml) | |
| 2 | 1 | 1 | IMP | 2 (mM) | |
| 3 | 1 | 1 | magnesium acetate | 10 (mM) | |
| 4 | 1 | 1 | N,N-bis-[2-hydroxyethyl]-2-aminoethanesulfonic acid | 10 (mM) | |
| 5 | 1 | 1 | EDTA | 0.1 (mM) | |
| 6 | 1 | 1 | sodium azide | 0.02 (%) |
