3GOZ
Crystal structure of the leucine-rich repeat-containing protein LegL7 from Legionella pneumophila. Northeast Structural Genomics Consortium target LgR148
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 67.196, 67.196, 155.420 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.598 - 2.099 |
| R-factor | 0.2084 |
| Rwork | 0.205 |
| R-free | 0.26200 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.930 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXDE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.099 | 2.099 |
| Rmerge | 0.063 | 0.529 |
| Number of reflections | 91532 | |
| <I/σ(I)> | 27.69 | 1.98 |
| Completeness [%] | 99.6 | 99 |
| Redundancy | 5.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 18% PEG 3350, 4% Ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
