3GOU
Crystal structure of dog (Canis familiaris) hemoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.435, 86.270, 130.565 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.510 - 3.000 |
| R-factor | 0.20502 |
| Rwork | 0.202 |
| R-free | 0.25623 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fhj |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.525 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.510 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 12084 | |
| <I/σ(I)> | 3.4 | 1.1 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 4.62 | 4.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 298 | phosphate buffer, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
