3GOJ
Barium bound to the Holliday sequence d(CCGGCGCCGG)4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | OXFORD SAPPHIRE CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.243, 24.241, 37.281 |
| Unit cell angles | 90.00, 110.17, 90.00 |
Refinement procedure
| Resolution | 15.615 - 2.600 |
| R-factor | 0.241 |
| Rwork | 0.236 |
| R-free | 0.34700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p4y |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.903 |
| Data reduction software | CrysalisPro (Red) |
| Data scaling software | CrysalisPro (Red) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.615 | 2.668 |
| High resolution limit [Å] | 2.601 | 2.601 |
| Rmerge | 0.058 | 0.193 |
| Number of reflections | 1757 | |
| <I/σ(I)> | 2.18 | |
| Completeness [%] | 98.6 | 98.6 |
| Redundancy | 2.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | BaCl2, MPD, NaCacodylate , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | BaCl2 | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | Na Cacodylate | ||
| 4 | 1 | 2 | BaCl2 | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | Na Cacodylate |
