3GOG
Guanine riboswitch A21G,U75C mutant bound to 6-chloroguanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-01-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.510, 35.120, 41.760 |
| Unit cell angles | 90.00, 90.57, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.100 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 11321 | |
| <I/σ(I)> | 8.5 | 3.5 |
| Completeness [%] | 98.8 | 97.6 |
| Redundancy | 4.91 | 4.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 25 % PEG 3K, 640 mM ammonium acetate, 12 mM cobalt hexammine, 10 mM K+, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3K | ||
| 2 | 1 | 1 | ammonium acetate | ||
| 3 | 1 | 1 | cobalt hexammine | ||
| 4 | 1 | 1 | K+ | ||
| 5 | 1 | 2 | PEG 3K | ||
| 6 | 1 | 2 | ammonium acetate | ||
| 7 | 1 | 2 | cobalt hexammine | ||
| 8 | 1 | 2 | K+ |
