3GO9
Predicted insulinase family protease from Yersinia pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.890, 80.230, 66.620 |
| Unit cell angles | 90.00, 99.34, 90.00 |
Refinement procedure
| Resolution | 42.300 - 1.620 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.20300 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.693 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.500 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.085 | 0.769 |
| Number of reflections | 56782 | |
| <I/σ(I)> | 32.233 | 2.81 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.02 M zinc chloride, 0.1 M ytrium chloride, 20% PEG-3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
