3GO6
Crystal Structure of M. tuberculosis ribokinase (Rv2436) in complex with ribose and AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.424, 117.900, 91.571 |
| Unit cell angles | 90.00, 113.93, 90.00 |
Refinement procedure
| Resolution | 48.170 - 1.980 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.170 | 50.000 | 2.050 |
| High resolution limit [Å] | 1.980 | 4.260 | 1.980 |
| Rmerge | 0.039 | 0.026 | 0.139 |
| Number of reflections | 52512 | ||
| <I/σ(I)> | 29.146 | ||
| Completeness [%] | 99.8 | 98.2 | 100 |
| Redundancy | 3.8 | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 295 | 0.2 M Sodium/potassium phosphate, 0.1 M Bis-Tris propane, 16% PEG 3350, pH 6.5, vapor diffusion, temperature 295K |
