3GO4

Crystal structure of a duf574 family protein (sav_2177) from streptomyces avermitilis ma-4680 at 1.80 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-22
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837
Spacegroup name	C 1 2 1
Unit cell lengths	94.637, 49.439, 77.811
Unit cell angles	90.00, 123.80, 90.00

Refinement procedure

Resolution	27.482 - 1.800
R-factor	0.202
Rwork	0.200
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3giw
RMSD bond length	0.015
RMSD bond angle	1.630
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.482	27.482	1.860
High resolution limit [Å]	1.800	3.870	1.800
Rmerge	0.099	0.048	0.478
Number of reflections	26949	5309	4709
<I/σ(I)>		13.7	1.6
Completeness [%]	95.3	96.2	92.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	NANODROP, 0.2M Sodium chloride, 2.0M Ammonium sulfate, 0.1M Sodium cacodylate pH 6.5, Additive: 0.001 M S-adenosyl-L-homocysteine, VAPOR DIFFUSION, SITTING DROP, temperature 277K