3GNM
The crystal structure of the JAA-F11 monoclonal antibody Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2007-05-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 94.210, 94.210, 94.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.18878 |
| Rwork | 0.185 |
| R-free | 0.26255 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1clz |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.944 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.096 | 0.447 |
| Number of reflections | 25154 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 99.0 | 97.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 294 | 26% PEG 5000, 50 mM LiBr, 50 mM NaCitrate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
