3GNG
P21B crystal structure of R1-R7 of Murine MVP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.776, 60.087, 68.628 |
| Unit cell angles | 90.00, 95.34, 90.00 |
Refinement procedure
| Resolution | 29.700 - 3.000 |
| R-factor | 0.25844 |
| Rwork | 0.257 |
| R-free | 0.28703 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gf5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.768 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.077 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 8896 | |
| Completeness [%] | 98.9 | 95.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 20% PEG 5000, 0.1M Sodium Citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
