3GNF
P1 Crystal structure of the N-terminal R1-R7 of murine MVP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.562, 51.892, 79.338 |
| Unit cell angles | 101.69, 92.28, 99.41 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.23952 |
| Rwork | 0.238 |
| R-free | 0.27622 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gf5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.264 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.031 |
| Number of reflections | 26128 |
| <I/σ(I)> | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 20%PEG 5000, 0.1M Sodium Citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
