3GN6

Crystal structure of CT0912, ORFan protein from Chlorobium tepidum with a ferredoxin-like domain repeat (NP_661805.1) from CHLOROBIUM TEPIDUM TLS at 1.80 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-17
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 21 21 2
Unit cell lengths	121.268, 210.205, 57.984
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.829 - 1.800
R-factor	0.164
Rwork	0.163
R-free	0.19200
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.486
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.829	23.421	1.860
High resolution limit [Å]	1.800	3.870	1.800
Rmerge	0.079	0.044	0.749
Number of reflections	137561	14192	12728
<I/σ(I)>	9.63	24.1	1.7
Completeness [%]	99.5	97.6	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2430M magnesium acetate, 13.6000% polyethylene glycol 3350, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K