3GN5
Structure of the E. coli protein MqsA (YgiT/b3021)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-08 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.054, 30.965, 75.542 |
| Unit cell angles | 90.00, 106.60, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.150 |
| R-factor | 0.18475 |
| Rwork | 0.182 |
| R-free | 0.23339 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 3GA8 and 3FMY |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.225 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 15349 | |
| <I/σ(I)> | 12.9 | 4.85 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 0.1M Bis-Tris, 0.2M magnesium chloride hexahydrate, 25% PEG 3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
