3GMW
Crystal Structure of Beta-Lactamse Inhibitory Protein-I (BLIP-I) in Complex with TEM-1 Beta-Lactamase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-08-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.418, 48.757, 106.137 |
| Unit cell angles | 103.24, 91.29, 90.18 |
Refinement procedure
| Resolution | 25.000 - 2.100 |
| R-factor | 0.1946 |
| Rwork | 0.192 |
| R-free | 0.23400 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.970 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 105.410 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.111 | |
| Number of reflections | 49303 | |
| <I/σ(I)> | 14.438 | |
| Completeness [%] | 94.7 | 86.5 |
| Redundancy | 5.4 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop vapor batch | 294 | 10 mg/ml of each protein in 50 mM TrisCl, pH 8.0, 0.05 M NaCl mixed 1:1 with 0.2 M potassium phosphate, pH 4.7, 20% PEG 3350, hanging drop vapor batch, temperature 294K |
