3GMT
Crystal structure of adenylate kinase from burkholderia pseudomallei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-31 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.230, 66.000, 63.760 |
| Unit cell angles | 90.00, 113.69, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.238 |
| Rwork | 0.235 |
| R-free | 0.29500 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.521 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.072 | 0.618 |
| Number of reflections | 27703 | |
| <I/σ(I)> | 17.02 | 3.1 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 7.4 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 290 | HAMPTON SCREEN, B5: 30% PEG 4000, 200MM LI2SO4, 100MM TRIS PH 8,, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
