3GME
Crystal Structure of Polynucleotide Phosphorylase in complex with RNase E and manganese
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.07 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 158.574, 158.574, 156.118 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.910 - 2.400 |
| R-factor | 0.28621 |
| Rwork | 0.286 |
| R-free | 0.29758 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.765 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.910 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.125 | 0.432 |
| Number of reflections | 27938 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 96.7 | 96.7 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 2.5 M NaCl, 9 % w/v PEG 6000, 20 mM Na- citrate, 20 mM manganese acetate , VAPOR DIFFUSION |
