3GM8
Crystal structure of a beta-glycosidase from Bacteroides vulgatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 62.754, 193.348, 190.667 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.229 |
| Rwork | 0.226 |
| R-free | 0.28700 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.572 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.769 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.144 | 0.465 |
| Total number of observations | 32873 | |
| Number of reflections | 45017 | |
| <I/σ(I)> | 8.4 | 2.9 |
| Completeness [%] | 98.3 | 99.2 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 294 | 100mM Tris HCl pH 8.0, 28% PEG 3350, 150mM ammonium sulfate, vapor diffusion, temperature 294K |
