3GLM
Glutaconyl-coA decarboxylase A subunit from Clostridium symbiosum co-crystallized with crotonyl-coA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.80150 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.500, 167.450, 138.110 |
| Unit cell angles | 90.00, 89.98, 90.00 |
Refinement procedure
| Resolution | 36.760 - 2.500 |
| R-factor | 0.19384 |
| Rwork | 0.193 |
| R-free | 0.23006 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.201 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 36.761 | 36.760 | 2.640 |
| High resolution limit [Å] | 2.500 | 7.910 | 2.500 |
| Rmerge | 0.097 | 0.037 | 0.464 |
| Total number of observations | 6929 | 27419 | |
| Number of reflections | 79234 | ||
| <I/σ(I)> | 6.055 | 9.2 | 1.1 |
| Completeness [%] | 99.6 | 97.7 | 98.9 |
| Redundancy | 2.7 | 2.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | co-crystallized with crotonyl-CoA, VAPOR DIFFUSION, HANGING DROP |
