3GLL
Crystal structure of Polynucleotide Phosphorylase (PNPase) core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 159.153, 159.153, 157.508 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.390 - 2.700 |
| R-factor | 0.27245 |
| Rwork | 0.271 |
| R-free | 0.30572 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.746 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.760 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.125 | 0.432 |
| Number of reflections | 19414 | |
| <I/σ(I)> | 9.2 | 1.8 |
| Completeness [%] | 96.7 | 96.7 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2 M ammonium nitrate, 20 % w/v PEG 3350, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
