3GLE
Crystal Structure of the Major Pilin from Streptococcus pyogenes N168A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-28 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.712, 50.869, 123.200 |
| Unit cell angles | 90.00, 104.27, 90.00 |
Refinement procedure
| Resolution | 18.000 - 2.190 |
| R-factor | 0.20879 |
| Rwork | 0.205 |
| R-free | 0.27783 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b2n |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.351 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0078) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.190 | 2.200 |
| Number of reflections | 40313 | |
| <I/σ(I)> | 38.7 | 6.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10.4 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2M MOPS/KOH pH7.5, 21% PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 291.0K |
