3GL1
Crystal structure of ATPase domain of Ssb1 chaperone, a member of the HSP70 family, from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.543, 54.504, 90.623 |
| Unit cell angles | 90.00, 104.36, 90.00 |
Refinement procedure
| Resolution | 37.700 - 1.920 |
| R-factor | 0.16672 |
| Rwork | 0.164 |
| R-free | 0.21776 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yuw |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.565 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.700 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.077 | 0.540 |
| Number of reflections | 54607 | |
| <I/σ(I)> | 18.982 | 2.11 |
| Completeness [%] | 98.3 | 86.7 |
| Redundancy | 4.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.2 M Magnesium chloride, 0.1 M Tris buffer, 25% PEG 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
