3GKF
Crystal Structure of E. coli LsrF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-01-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 78.501, 104.610, 171.673 |
| Unit cell angles | 89.88, 79.31, 89.61 |
Refinement procedure
| Resolution | 29.640 - 2.900 |
| R-factor | 0.21 |
| Rwork | 0.209 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gkf |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.317 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 6.250 | 2.900 |
| Rmerge | 0.092 | 0.039 | 0.361 |
| Number of reflections | 113378 | ||
| <I/σ(I)> | 9.057 | ||
| Completeness [%] | 95.3 | 96.5 | 90.5 |
| Redundancy | 1.8 | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 4% PEG 400, 100 mM MgCl2, 2.3 M Ammonium Sulfate, pH 7.5, vapor diffusion, temperature 298K |
