3GJU

Crystal structure of a putative aminotransferase (mll7127) from mesorhizobium loti maff303099 at 1.55 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-18
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162, 0.97941, 0.97954
Spacegroup name	C 1 2 1
Unit cell lengths	113.224, 59.577, 85.337
Unit cell angles	90.00, 130.59, 90.00

Refinement procedure

Resolution	28.330 - 1.550
R-factor	0.14
Rwork	0.139
R-free	0.16800
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.533
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.330	28.330	1.610
High resolution limit [Å]	1.550	3.330	1.550
Rmerge	0.057	0.018	0.520
Number of reflections	61448	11810	11275
<I/σ(I)>		28.8	1.5
Completeness [%]	92.0	94.4	85.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	NANODROP, 59.1% 2-methyl-2,4-pentanediol, 0.1M Bicine pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K