3GJO
Crystal structure of human EB1 in complex with microtubule Tip localization signal peptide of MACF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-02-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0009 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.614, 44.896, 74.840 |
| Unit cell angles | 90.00, 98.57, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.500 |
| R-factor | 0.215 |
| Rwork | 0.213 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wu9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.131 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.500 | 2.680 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 10167 | |
| <I/σ(I)> | 9.94 | 3.43 |
| Completeness [%] | 96.1 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | 0.2M MAGNESIUM ACETATE, 20% PEG 3350, pH 7.40, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
