3GJD
Crystal Structure of LeuT with bound OG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-03-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.510, 86.570, 81.290 |
| Unit cell angles | 90.00, 95.63, 90.00 |
Refinement procedure
| Resolution | 29.553 - 2.000 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a65 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.919 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 39607 | |
| <I/σ(I)> | 15.5 | 3.75 |
| Completeness [%] | 97.0 | 96.2 |
| Redundancy | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 150mM NaCl, 100mM Hepes, 23% PEG 550 MME, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
