3GJC
Crystal Structure of the E290S mutant of LeuT with bound OG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-08 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9081 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.790, 93.510, 87.460 |
| Unit cell angles | 90.00, 94.22, 90.00 |
Refinement procedure
| Resolution | 29.204 - 2.800 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a65 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.904 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 32597 | |
| <I/σ(I)> | 9.06 | 1.96 |
| Completeness [%] | 97.8 | 98.6 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 150mM NaCl, 100mM Hepes, 23% PEG 550 MME, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
