3GJ2
Photoactivated state of PA-GFP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.714, 87.069, 144.862 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.660 - 1.900 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.24800 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3gj1 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.108 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.514 |
| Number of reflections | 83384 | |
| <I/σ(I)> | 24.652 | |
| Completeness [%] | 97.9 | 92.7 |
| Redundancy | 5.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 18% PEG 3550, 0.1 M Tris-HCl, 0.2 M Calcium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
