3GIW
CRYSTAL STRUCTURE OF a DUF574 family protein (SAV_2177) FROM STREPTOMYCES AVERMITILIS MA-4680 AT 1.45 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-08 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837, 0.97927 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.716, 49.669, 45.132 |
| Unit cell angles | 90.00, 94.72, 90.00 |
Refinement procedure
| Resolution | 27.186 - 1.450 |
| R-factor | 0.157 |
| Rwork | 0.156 |
| R-free | 0.17600 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.511 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.186 | 27.186 | 1.500 |
| High resolution limit [Å] | 1.450 | 3.120 | 1.450 |
| Rmerge | 0.036 | 0.023 | 0.333 |
| Number of reflections | 50240 | ||
| <I/σ(I)> | 13.4 | 31.1 | 2.5 |
| Completeness [%] | 94.8 | 96.3 | 85.9 |
| Redundancy | 4.063 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.0 M Na citrate, 0.1 M Sodium cacodylate pH 6.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
