3GI7

Crystal structure of a duf1311 family protein (pp0307) from pseudomonas putida kt2440 at 1.85 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-19
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162, 0.97964, 0.97949
Spacegroup name	P 41 21 2
Unit cell lengths	100.027, 100.027, 58.343
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.173 - 1.850
R-factor	0.179
Rwork	0.178
R-free	0.19900
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.486
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.5)
Phasing software	SHELX
Refinement software	REFMAC (5.5.0070)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.173	29.173	1.900
High resolution limit [Å]	1.850	8.270	1.850
Rmerge	0.065	0.034	0.692
Rmeas	0.070
Total number of observations		1960	13774
Number of reflections	25875
<I/σ(I)>	18.5	17.6	2.7
Completeness [%]	100.0	97.2	100
Redundancy	7.2	5.7	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.67	293	NANODROP, 2.10M Ammonium sulfate, 0.250M Potassium sodium tartrate, 0.1M Sodium citrate - citric acid pH 5.67, VAPOR DIFFUSION, SITTING DROP, temperature 293K