3GI7
Crystal structure of a duf1311 family protein (pp0307) from pseudomonas putida kt2440 at 1.85 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-19 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91162, 0.97964, 0.97949 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 100.027, 100.027, 58.343 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.173 - 1.850 |
R-factor | 0.179 |
Rwork | 0.178 |
R-free | 0.19900 |
Structure solution method | MAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.486 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.5) |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.173 | 29.173 | 1.900 |
High resolution limit [Å] | 1.850 | 8.270 | 1.850 |
Rmerge | 0.065 | 0.034 | 0.692 |
Rmeas | 0.070 | ||
Total number of observations | 1960 | 13774 | |
Number of reflections | 25875 | ||
<I/σ(I)> | 18.5 | 17.6 | 2.7 |
Completeness [%] | 100.0 | 97.2 | 100 |
Redundancy | 7.2 | 5.7 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.67 | 293 | NANODROP, 2.10M Ammonium sulfate, 0.250M Potassium sodium tartrate, 0.1M Sodium citrate - citric acid pH 5.67, VAPOR DIFFUSION, SITTING DROP, temperature 293K |