3GI1

Crystal Structure of the laminin-binding protein Lbp of Streptococcus pyogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-08-20
Detector	MAR345DTB
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	42.624, 92.163, 70.614
Unit cell angles	90.00, 106.27, 90.00

Refinement procedure

Resolution	27.982 - 2.450
R-factor	0.19
Rwork	0.186
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1toa 1k0f 1pq4 1xvl 2o1e 3hh8
RMSD bond length	0.015
RMSD bond angle	1.560
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.33)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.984	29.090	2.530
High resolution limit [Å]	2.400	7.590	2.400
Rmerge	0.098	0.042	0.597
Total number of observations		4771	21627
Number of reflections	20587
<I/σ(I)>	6.861	13.9	1.2
Completeness [%]	99.9	97.9	100
Redundancy	7.3	7.1	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	291	0.7ul of 37 mg/ml Lbp in PBS, pH7.4 + 0.002% Na-azide + 0.7ul of 30% PEG1500, VAPOR DIFFUSION, SITTING DROP, temperature 291K