3GHY
Crystal structure of a putative ketopantoate reductase from Ralstonia solanacearum MolK2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2009-02-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 43 |
| Unit cell lengths | 70.648, 70.648, 145.409 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.23296 |
| Rwork | 0.231 |
| R-free | 0.27786 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.267 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.495 | |
| Number of reflections | 47980 | |
| <I/σ(I)> | 12.3 | 2 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 5.7 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 0.1M Bis-Tris pH 6.5, 30% PEG MME 550, 0.05M Calcium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
