3GHJ
Crystal structure from the mobile metagenome of Halifax Harbour Sewage Outfall: Integron Cassette Protein HFX_CASS4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-12 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 51.001, 66.391, 80.822 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.319 - 1.471 |
| R-factor | 0.169 |
| Rwork | 0.168 |
| R-free | 0.18300 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.074 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX (AUTOSOL) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.074 | 0.344 |
| Number of reflections | 23542 | |
| <I/σ(I)> | 37.28 | 4.07 |
| Completeness [%] | 99.3 | 95.7 |
| Redundancy | 8.1 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 294 | 0.2 M Ammonium sulfate, 25% PEG 4000, 0.1 M Na acetate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
