3GHF
Crystal structure of the septum site-determining protein minC from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-30 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 48.380, 48.380, 85.127 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.241 |
| Rwork | 0.238 |
| R-free | 0.29500 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.598 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.495 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.077 | 0.407 |
| Total number of observations | 9406 | |
| Number of reflections | 6234 | |
| <I/σ(I)> | 16.8 | 4.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.5 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 3.5 | 294 | 100mM Sodium citrate pH 3.5, 6% Glycerol, 22% PEG 3350, VAPOR DIFFUSION, temperature 294K |
