3GHD
Crystal structure of a cystathionine beta-synthase domain protein fused to a Zn-ribbon-like domain
Replaces: 3FIOExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-20 |
| Detector | unsupported-m300 |
| Wavelength(s) | 0.97941 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 54.153, 73.162, 35.264 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.770 - 1.810 |
| R-factor | 0.19455 |
| Rwork | 0.192 |
| R-free | 0.24108 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.807 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.094 | 0.202 |
| Number of reflections | 11994 | |
| <I/σ(I)> | 14.6 | 5.84 |
| Completeness [%] | 89.5 | 61 |
| Redundancy | 6.2 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297 | 0.2M Ammonium Chloride,20%PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
