3GGN
Crystal structure of DR_A0006 from Deinococcus radiodurans. Northeast Structural Genomics Consortium Target DrR147D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.498, 56.286, 51.277 |
| Unit cell angles | 90.00, 106.23, 90.00 |
Refinement procedure
| Resolution | 37.060 - 2.000 |
| R-factor | 0.242 |
| Rwork | 0.242 |
| R-free | 0.27400 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.078 | 0.319 |
| Number of reflections | 35348 | |
| <I/σ(I)> | 14.8 | |
| Completeness [%] | 98.6 | 96.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 6 | 277 | 0.1M KH2PO4, 0.1M MES pH 6.0, 40% PEG 4000, MICROBATCH UNDER OIL, temperature 277.0K |
