3GGE

Crystal structure of the PDZ domain of PDZ domain-containing protein GIPC2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-30
Detector	MAR225 CCD
Wavelength(s)	0.8800
Spacegroup name	P 41 2 2
Unit cell lengths	83.510, 83.510, 160.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.760 - 2.600
R-factor	0.22504
Rwork	0.223
R-free	0.26456
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kwa
RMSD bond length	0.015
RMSD bond angle	1.458
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0085)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.370	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.112	0.767
Number of reflections	18077
<I/σ(I)>	12.3	2.5
Completeness [%]	100.0	100
Redundancy	6.3	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277.15	2M ammonium sulfate; 2.5% v/v propanol; , pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K