3GG8
Crystal structure of the Toxoplasma gondii Pyruvate Kinase N terminal truncated
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-17 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.023, 92.311, 112.443 |
| Unit cell angles | 90.00, 105.57, 90.00 |
Refinement procedure
| Resolution | 42.450 - 2.210 |
| R-factor | 0.20923 |
| Rwork | 0.206 |
| R-free | 0.26516 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eoe |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.282 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.088 | 0.913 |
| Number of reflections | 105806 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 97.9 | 96.3 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 5 % PEG 3350, 0.1 M Succinic Acid, pH 7.0, 3 mM Chembridge #5175181, 20% glycerol, Vapor diffusion, hanging drop |
