3GG7
Crystal structure of an uncharacterized metalloprotein from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-27 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 41 |
| Unit cell lengths | 47.207, 47.207, 97.787 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.184 |
| Rwork | 0.183 |
| R-free | 0.19900 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.365 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.207 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.067 | 0.479 |
| Total number of observations | 51755 | |
| Number of reflections | 34218 | |
| <I/σ(I)> | 17.9 | 3.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.5 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 294 | 100mM Hepes pH 7.5, 30% MPD, 500mM ammonium sulfate, vapor diffusion, temperature 294K |
