3GG6
Crystal structure of the NUDIX domain of human NUDT18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2008-12-11 |
| Detector | ADSC QUANTUM Q315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.660, 60.130, 76.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.801 - 2.100 |
| R-factor | 0.1699 |
| Rwork | 0.164 |
| R-free | 0.22560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b0v |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.816 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.161 | 0.557 |
| Number of reflections | 8985 | |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 7.88 | 5.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 293 | Tri-sodium citrate dihydrate 0.1M pH 5.5, PEG3000 20% w/v, 2mM 2 deoxyguanosine, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
