3GG4
The crystal structure of glycerol kinase from Yersinia pseudotuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-02-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.789, 110.393, 80.952 |
| Unit cell angles | 90.00, 99.13, 90.00 |
Refinement procedure
| Resolution | 36.004 - 2.000 |
| R-factor | 0.2006 |
| Rwork | 0.198 |
| R-free | 0.24550 |
| Structure solution method | PHENIX AUTOSOl |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX (AUTOSOL) |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.810 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.071 | 0.448 |
| Number of reflections | 90545 | |
| <I/σ(I)> | 3.4 | |
| Completeness [%] | 93.0 | 67.9 |
| Redundancy | 7.1 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.2 M Magnesium chloride hexahydrate, 0.1 M BIS-TRIS pH 5.5, 25% w/v PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
