3GFR
Structure of YhdA, D137L variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.8148 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.491, 56.080, 64.176 |
| Unit cell angles | 84.06, 77.02, 74.49 |
Refinement procedure
| Resolution | 19.971 - 2.403 |
| R-factor | 0.202 |
| Rwork | 0.199 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nni |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.614 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 24878 | |
| <I/σ(I)> | 3.2 | |
| Completeness [%] | 95.0 | 88.8 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch crystallization | 6.5 | 298 | 0.1 M Bis-Tris pH 6.5, 20% w/v PEG MME 5000, batch crystallization, temperature 298K |
