3GFQ
Structure of YhdA, K109L variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.8010 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 47.543, 66.238, 220.748 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.097 - 2.996 |
| R-factor | 0.225 |
| Rwork | 0.222 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nni |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.657 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.100 | 3.050 |
| High resolution limit [Å] | 2.996 | 2.996 |
| Number of reflections | 14641 | |
| <I/σ(I)> | 1.5 | |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 4.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch crystallization | 7.5 | 298 | 0.2 M Ammonium Sulfate, 0.1 M HEPES pH 7.5, 25% w/v PEG 3350, batch crystallization, temperature 298K |
