3GFO
Structure of cbiO1 from clostridium perfringens: Part of the ABC transporter complex cbiONQ.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.701, 57.578, 41.245 |
| Unit cell angles | 90.00, 103.39, 90.00 |
Refinement procedure
| Resolution | 40.130 - 2.300 |
| R-factor | 0.209 |
| Rwork | 0.206 |
| R-free | 0.26300 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.456 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.084 | 0.395 |
| Number of reflections | 11602 | |
| <I/σ(I)> | 17.135 | 2.61 |
| Completeness [%] | 99.6 | 97.3 |
| Redundancy | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1 M MES pH 6.5, 30% Jeffamine M-600 pH 7 0.05M Cesium Chloride , VAPOR DIFFUSION, SITTING DROP, temperature 298K |
