3GFI
Crystal structure of ST1710 complexed with its promoter DNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2008-11-27 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97896, 0.97930, 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 94.440, 106.730, 82.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.750 - 2.100 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.28000 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 24231 | |
| Completeness [%] | 99.0 | 98.6 |
| Redundancy | 9.6 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.8 | 293 | 30% 2-methyl-2,4-pentanediol (MPD) 0.02M Calcium chloride dehydrate, 0.1M sodium trihydrate, pH 3.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | Calcium chloride dehydrate | ||
| 3 | 1 | 1 | sodium trihydrate | ||
| 4 | 1 | 2 | MPD | ||
| 5 | 1 | 2 | Calcium chloride dehydrate | ||
| 6 | 1 | 2 | sodium trihydrate |
