3GF5

Crystal structure of the P21 R1-R7 N-terminal domain of murine MVP

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2008-05-12
Detector	PSI PILATUS 6M
Wavelength(s)	0.979,0.976
Spacegroup name	P 1 21 1
Unit cell lengths	36.523, 98.892, 140.581
Unit cell angles	90.00, 97.18, 90.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.2333
Rwork	0.232
R-free	0.26691
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.912
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.565
High resolution limit [Å]	2.500	2.500
Rmerge	0.071
Number of reflections	33339
<I/σ(I)>	10.1
Completeness [%]	96.0	81.84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	277	20% PEG 5000, 0.1M Sodium citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K