3GEM
Crystal structure of short-chain dehydrogenase from Pseudomonas syringae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.198, 73.770, 162.168 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.800 - 1.830 |
| R-factor | 0.151 |
| Rwork | 0.149 |
| R-free | 0.18200 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.601 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.800 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.103 | 0.795 |
| Number of reflections | 78827 | |
| <I/σ(I)> | 26.617 | 2.71 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M HEPES buffer, 0.2 M Ammonium sulfate, 27% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
