3GEA
Donor strand complemented FaeG monomer of F4 variant ad
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0723 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 123.965, 123.965, 95.886 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.677 - 1.699 |
| R-factor | 0.1604 |
| Rwork | 0.159 |
| R-free | 0.18280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j6g |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.537 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.677 | 1.790 |
| High resolution limit [Å] | 1.699 | 1.699 |
| Rmerge | 0.115 | 0.664 |
| Number of reflections | 93586 | |
| <I/σ(I)> | 16.5 | 3.7 |
| Completeness [%] | 100.0 | 99.3 |
| Redundancy | 10.5 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 1.8M ammonium sulphate, 0.1M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
