3GDQ
Crystal structure of the human 70kDa heat shock protein 1-like ATPase domain in complex with ADP and inorganic phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2008-12-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.070, 70.700, 97.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.830 - 1.800 |
| R-factor | 0.17224 |
| Rwork | 0.171 |
| R-free | 0.20249 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ba0 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.367 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 45400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 26% PEG monomethyl ether 2000, 0.1M Tris, 0.2M trimethylamine n-oxide, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
